La regeneración osteocondral (OC) presenta varias limitaciones en el campo de la cirugía ortopédica debido a la complejidad del tejido OC (regeneración simultanes del cartílago articular y hueso subcondral).
En este estudio se han preparado soportes biofuncionales compuestos de una capa de fibroina entrecruzada con peroxidasa con una estructura similar al cartílago y una capa similar al hueso compuets de fosfato tricalcico dopado con iones Zn y Sr. Se logró una distribución de porosidad homogénea en todos los soportes, como se muestra en el análisis de tomografía microcomputada. Los soportes bicapa dopados con iones presentaron un módulo de compresión húmeda (226.56 ± 60.34 kPa) y propiedades mecánicas dinámicas (de 403.56 ± 111.62 a 593.56 ± 206.90 kPa). La formación de cristales de apatita, después de la inmersión en fluido corporal simulado (SBF), se observó en las capas similares al hueso subcondra. Se cultivaron osteoblastos humanos (hOBs) y condrocitos articulares humanos (hACs) sobre los soportes bicapa. ambos tipos de células mostraron una buena adhesión y proliferación, así como una integración adecuada de las diferentes regiones. Los osteoblastos produjeron una matriz extracelular mineralizada (ECM) en las capas similares al hueso subcondral, y los condrocitos mostraron deposición de GAG. El perfil de expresión génica fue diferente en las distintas zonas de los soportes bicapa, mientras que regiones intermedias mostraron expresión génica de condrocitos pre-hipertróficos. Este estudio mostró que los soportes bicapa propuestos permitieron una estimulación específica de las células condrogénicas y osteogénicas en co-cultivo junto con la formación de una interfaz de tejido tipo osteocondral. Por lo tanto, la adaptabilidad estructural, las propiedades mecánicas adecuadas y el rendimiento biológico de estos soportes hacen que estos sistemas sean adecuados la regeneración de defectos osteocondrales.
3729048
{3729048:G52VSA9M}
nature
50
1
1
title
574
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