Supercritical CO2 assisted bioMOF drug encapsulation and functionalization for delivery with a synergetic therapeutic value

Biomateriales

Despite the impressive characteristics of biological metal organic frameworks (bioMOFs) for their use as drug delivery systems (DDs), there are still some parameters related to their structural stability and processing routes that have decelerated their realistic application in this field. Both drawbacks are unraveled in this work for the microporous bioMOF CaSyr-1 by using supercritical CO2 (scCO2) to load the bioMOF with the anti-tubercular isoniazid (INH) drug, and functionalize its external surface with a hydrophobic protective layer of stearate (S). The hydrophobicized CaSyr-1(INH)/S vehicle is further coated with a neutral surfactant (PS60) to enhance the wettability of the system. In vitro tests, related to drug carrier biocompatibility and drug release in body simulated fluids, are performed to demonstrate potential prospective of the designed DDs in pharmacy. The synthetized product displayed total biocompatibility even at high concentrations, and the particle size and dissolution rate showed to be adequate for pulmonary administration.

1.
Rosado, A. et al. Supercritical CO2 assisted bioMOF drug encapsulation and functionalization for delivery with a synergetic therapeutic value. The Journal of Supercritical Fluids 106452 (2024) http://doi.org/10.1016/j.supflu.2024.106452. Cite

BioMOF@cellulose Glycerogel Scaffold with Multifold Bioactivity: Perspective in Bone Tissue Repair

Biomateriales

The development of new biomaterials for musculoskeletal tissue repair is currently an important branch in biomedicine research. The approach presented here is centered around the development of a prototypic synthetic glycerogel scaffold for bone regeneration, which simultaneously features therapeutic activity. The main novelty of this work lies in the combination of an open meso and macroporous nanocrystalline cellulose (NCC)-based glycerogel with a fully biocompatible microporous bioMOF system (CaSyr-1) composed of calcium ions and syringic acid. The bioMOF framework is further impregnated with a third bioactive component, i.e., ibuprofen (ibu), to generate a multifold bioactive system. The integrated CaSyr-1(ibu) serves as a reservoir for bioactive compounds delivery, while the NCC scaffold is the proposed matrix for cell ingrowth, proliferation and differentiation. The measured drug delivery profiles, studied in a phosphate-buffered saline solution at 310 K, indicate that the bioactive components are released concurrently with bioMOF dissolution after ca. 30 min following a pseudo-first-order kinetic model. Furthermore, according to the semi-empirical Korsmeyer-Peppas kinetic model, this release is governed by a case-II mechanism, suggesting that the molecular transport is influenced by the relaxation of the NCC matrix. Preliminary in vitro results denote that the initial high concentration of glycerol in the NCC scaffold can be toxic in direct contact with human osteoblasts (HObs). However, when the excess of glycerol is diluted in the system (after the second day of the experiment), the direct and indirect assays confirm full biocompatibility and suitability for HOb proliferation.

1.
Rosado, A. et al. BioMOF@cellulose Glycerogel Scaffold with Multifold Bioactivity: Perspective in Bone Tissue Repair. Gels 10, 631 (2024). Cite

WP Twitter Auto Publish Powered By : XYZScripts.com