TY - JOUR TI - α-Tocopheryl Succinate-Based Polymeric Nanoparticles for the Treatment of Head and Neck Squamous Cell Carcinoma AU - Sánchez-Rodríguez, Carolina AU - Palao-Suay, Raquel AU - Rodrigáñez, Laura AU - Aguilar, María AU - Martín-Saldaña, Sergio AU - San Román, Julio AU - Sanz-Fernández, Ricardo AU - Sánchez-Rodríguez, Carolina AU - Palao-Suay, Raquel AU - Rodrigáñez, Laura AU - Aguilar, María Rosa AU - Martín-Saldaña, Sergio AU - San Román, Julio AU - Sanz-Fernández, Ricardo T2 - Biomolecules AB - The aim of this work is to study, in an in vitro head and neck squamous cell carcinomas model the anti-angiogenic and anti-migratory properties of self-assembled polymeric nanoparticles (NPs) with demonstrated selective anticancer activity. The NPs are based on α-tocopheryl succinate (α-TOS) encapsulated in the hydrophobic core of the NPs. We analyzed the effect of the newly synthetized α-TOS-loaded NPs in proliferating endothelial cells and hypopharynx carcinoma squamous cells and measured markers of angiogenesis, apoptosis and reactive oxygen species (ROS). α-TOS-loaded NPs suppressed angiogenesis by inducing accumulation of ROS and inducing apoptosis of proliferating endothelial cells. These NPs also decrease the number and quality of capillary-like tubes in an in vitro three-dimensional (3D) experiment, decrease the production of the pro-angiogenic vascular endothelial growth factor and down-regulate the expression of its receptor. The anti-migratory efficacy of α-TOS is corroborated in hypopharynx carcinoma cells by decreasing the secretion of matrix metalloproteases 2 and 9 (MMP-2 and MMP-9) and inhibiting cell migration. These results confirm that α-TOS-based NPs not only present anticancer properties, but also antiangiogenic properties, therefore making them promising candidates for multi-active combinatorial anticancer therapy. DA - 2018/09/19/ PY - 2018 DO - 10.3390/biom8030097 DP - www.mdpi.com VL - 8 IS - 3 SP - 97 LA - en UR - http://www.mdpi.com/2218-273X/8/3/97 Y2 - 2018/09/20/08:49:37 KW - angiogenesis KW - hypopharynx carcinoma squamous cells KW - migration KW - polymeric nanoparticle KW - proliferating endothelial cells KW - α-tocopheryl succinate ER -