TY - JOUR TI - Otoprotective properties of 6 alpha-methylprednisolone-loaded nanoparticles against cisplatin: In vitro and in vivo correlation AU - Martin-Saldana, Sergio AU - Palao-Suay, Raquel AU - Trinidad, Almudena AU - Rosa Aguilar, Maria AU - Ramirez-Camacho, Rafael AU - San Roman, Julio T2 - Nanomedicine-Nanotechnology Biology and Medicine AB - 6 alpha-Methylprednisolone-loaded surfactant-free nanoparticles have been developed to palliate cisplatin ototoxicity. Nanoparticles were based on two different amphiphilic pseudo-block copolymers obtained by free radical polymerization and based on N-vinyl pyrrolidone and a methacrylic derivative of a-tocopheryl succinate or alpha-tocopherol. Copolymers formed spherical nanoparticles by nanoprecipitation in aqueous media that were able to encapsulate 6 alpha-methylprednisolone in their inner core. The obtained nanovehicles were tested in vitro using HEI-OC1 cells and in vivo in a murine model. Unloaded nanoparticles were not able to significantly reduce the cisplatin ototoxicity. Loaded nanoparticles reduced cisplatin-ototoxicity in vitro being more active those based on the methacrylic derivative of vitamin E, due to their higher encapsulation efficiency. This formulation was able to protect hair cells in the base of the cochlea, having a positive effect in the highest frequencies tested in a murine model. A good correlation between the in vitro and the in vivo experiments was found. From the Clinical Editor: Cisplatin is a commonly used chemotherapeutic agent against many cancers clinically. However, one of the significant side-effects remains ototoxicity. Here, the authors presented their data on using 6 alpha-methylprednisolone-loaded nanoparticles in the reduction of ototoxicity in in-vitro and in-vivo experiments. Early promising results should enable further refinement of adopting this new approach in future experiments. (C) 2016 Elsevier Inc. All rights reserved. DA - 2016/05// PY - 2016 DO - 10.1016/j.nano.2015.12.367 DP - Web of Science VL - 12 IS - 4 SP - 965 EP - 976 J2 - Nanomed.-Nanotechnol. Biol. Med. LA - English SN - 1549-9634 ST - Otoprotective properties of 6 alpha-methylprednisolone-loaded nanoparticles against cisplatin KW - Cisplatin KW - Nanoparticles KW - Otoprotection KW - Vitamin E KW - alpha-Tocopheryl succinate KW - auditory cell-line KW - dexamethasone KW - drug-delivery KW - hearing-loss KW - hei-oc1 KW - induced apoptosis KW - induced ototoxicity KW - inner-ear disease KW - protection KW - system KW - vitamin-e ER -